Total Flavones of Selaginella uncinata (Desv.) Spring Inhibits Breast Cancer Cell Proliferation and Induces Apoptosis via Regulating microRNA-1269
نویسندگان
چکیده
Total flavones of Selaginella uncinata (Desv.) spring has inhibitory effects on cancer progression. The study aims to analyze the total breast cell tumor properties and possible mechanism. MDA-MB-231 cells were treated with or transfected anti-microRNA-negative control anti-microRNA-1269. In addition, microRNA-negative microRNA-1269 mimics-transfected exposed uncinata. Cell proliferation apoptosis investigated via counting kit-8, colony formation flow cytometry analysis. MicroRNA-1269, cleaved caspase-3 caspase-9 expression was determined through quantitative polymerase chain reaction Western blotting. After treatment, inhibition rate, protein levels increased, number colonies decreased, downregulated in a dose-dependent way. Relative MCF-10A cells, upregulated cells. transfection anti-microRNA-1269, rate but colony-forming ability decreased. treatment mimics uncinata, reduced promoted. can inhibit induce negatively modulating microRNA-1269.
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ژورنال
عنوان ژورنال: Indian Journal of Pharmaceutical Sciences
سال: 2023
ISSN: ['0250-474X', '1998-3743']
DOI: https://doi.org/10.36468/pharmaceutical-sciences.1149